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Microfabrication and microfluidics for 3D brain-on-chip

Microfabrication and microfluidics for 3D brain-on-chip. N2 - A revolution in cell culturing has been seen in the last two decades in which 3D culturing became the state-of the-art, with the goal to mimic the in-vivo situation more closely and allow for a physiologically more relevant study compared to the environment in a petri dish.

This revolution, however, has not been fully incorporated in the field of in-vitro neuroscience and neither in commercial activities, yet. In this work an attempt has been made to make such a 3D environment available for a conventional planar microelectrode array MEA for in-vitro electrophysiology.

Microfabrication for Microfluidics - Sang-Joon John Lee, Narayanan Sundararajan - Google книги

A reliable platform is realized by combining microfabrication and microfluidics towards a 3D brain-on-chip allowing for drug discovery and the study on diseases. A novel hybrid microbioreactor has been developed as a tool for hydrogel 3D cell culturing on a conventional MEA towards a more physiologically relevant model for the study on brain tissue.

Furthermore the hybrid bioreactor is explored to realize an on-chip biochemical readout from a 3D culture by a commercial microchip capillary electrophoresis CE system with contactless conductivity detection C4D. In conclusion, by combining microfabrication and microfluidics a platform has been realized towards a 3D brain-on-chip.

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The conventional microelectrode array principle is engaged to realize a 3D cell environment for the cells positioned at the sensing electrodes establishing the neuroelectronic interface and a microbioreactor add-on is realized allows for 3D culturing of the cells in between the electrodes neuronal network. Hereby a allowing for more physiological relevant studies with an electrophysiological readout. AB - A revolution in cell culturing has been seen in the last two decades in which 3D culturing became the state-of the-art, with the goal to mimic the in-vivo situation more closely and allow for a physiologically more relevant study compared to the environment in a petri dish.

In situ Microfabrication of valves with Flow Analysis

Microfabrication and microfluidics for 3D brain-on-chip Bart Schurink. Abstract A revolution in cell culturing has been seen in the last two decades in which 3D culturing became the state-of the-art, with the goal to mimic the in-vivo situation more closely and allow for a physiologically more relevant study compared to the environment in a petri dish.

Controlling the mass transport in the microchannels mostly depends on material used and channel geometry is the key parameter to improve flows speed, reaction sensitivity and surface robustness.


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As the flow type in the microfluidic channels is laminar, micro-mixers have been using to provide semi-turbulent flow inside the microchannels. In this study, microfluidic molds were fabricated by using 3D printing method and mixing phenomena was observed in different microplatforms with and without micro-mixer geometries to understand the underlying diffusion mechanism, which causes to mixing phenomena in the microchannel.

Microfluidics & Microfabrication Facility

Ejovoc Electronic Journal of Vocational Colleges. Year , Volume 8 , Issue 2, Pages - Zotero Mendeley EndNote. Abstract en tr Microfluidic devices are currently replacing their macroscopic counterparts in many applications. Keywords en tr microfluidics , diffusion , micromixer. References Akay, S.

An injectable alginate-based hydrogel for microfluidic applications. Carbohydrate Polymers, , — Microfluidic organs-on-chips.